Subcellular localization and release of human neutrophil gelatinase, confirming the existence of separate gelatinase-containing granules
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چکیده
منابع مشابه
Subcellular localization and release of human neutrophil gelatinase, confirming the existence of separate gelatinase-containing granules.
An e.l.i.s.a. was developed using specific polyclonal rabbit antibodies against human neutrophil gelatinase. This assay, in contrast to the functional assay, is independent of activation of gelatinase, and is specific for the detection of gelatinase in both its reduced and unreduced forms. Using this assay, we were able to demonstrate a difference between the subcellular localization of gelatin...
متن کاملHuman neutrophil gelatinase is a component of specific granules.
Previous investigators have proposed that gelatinase, a metalloproteinase found in neutrophils, is stored in a novel secretory compartment distinct from the two major granule populations, azurophilic and specific. To locate this proteinase in human neutrophils we reacted the cells for peroxidase and then applied monospecific polyclonal antibodies to human neutrophil gelatinase to immunolabel ul...
متن کاملHuman Neutrophil Granules and Secretory Vesicles Azurophil Granules Specific Granules Gelatinase Granules Secretory
neity simply that some proteins must be segregated to survive P inside the neutrophil, or for the neutrophil to survive, much OLYMORPHONUCLEAR leukocytes were discovered by Paul Ehrlich, when fixation and staining techniques as two-component glue has to be stored in two separate made it possible to identify the lobulated nucleus and the tubes, although both will be used at the same time? Whethe...
متن کاملExpression and subcellular localization of NRAMP1 in human neutrophil granules.
Mutations at the Nramp1 gene cause susceptibility to infections with intracellular pathogens. In human blood, polymorphonuclear (PMN) leukocytes are the most abundant site of NRAMP1 messenger RNA (mRNA) expression, suggesting that NRAMP1 plays an important role in the activity of these cells. By Northern blot analysis, NRAMP1 mRNA was only detected in most mature neutrophils from bone marrow (b...
متن کاملIsolation and characterization of gelatinase granules from human neutrophils.
We recently confirmed the existence of gelatinase granules as a subpopulation of peroxidase-negative granules by double-labeling immunogold electron microscopy on intact cells and by subcellular fractionation. Further characterization of gelatinase granules has been hampered by poor separation of specific and gelatinase granules on both two-layer Percoll gradients and sucrose gradients. We have...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1992
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2870603